AS 5013.10:2022 pdf free download – Food microbiology Method 10: Microbiology of the food chain-Horizontal method forthe detection, enumeration and serotyping of Salmonella— Detectionof Salmonella spp. (ISO 6579-1:2017,MOD)

AS 5013.10:2022 pdf free download – Food microbiology Method 10: Microbiology of the food chain-Horizontal method forthe detection, enumeration and serotyping of Salmonella— Detectionof Salmonella spp. (ISO 6579-1:2017,MOD).
This document specifies a horizontal method for the detection of Salmonella. It is applicable to
the following:
— products Intended for human consumption and the feeding of animals;
— environmental samples in the area of food production and food handling;
— samples From the primary production stage such as animal faeces, dust, and swabs.
With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and Salmonella Paratyphi, the procedure is described in Annex D.
The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella strains.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (Including any amendments) applies.
ISO 6887 (all parts), Microbiology offood and animal feed — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination
ISO 7218. Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological exominution.c
ISO 11133:2014, Microbiology of food, animal feed and water — Preparation, production. storage and performance testing of culture media
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminologwal databases for use in standardization at the following addresses:
— IEC Ekctropedla: available at http://www.electropcdia.org/
8.13.2 Preparation
Dissolve the sodium chloride In the water.
Adjust the pH, if necessary, so that after sterilization, it is 7,0 ± 0,2 at 25 °C.
Dispense the solution into flasks or tubes (6.12) of suitable capacity to obtain the portions necessary for the test.
Sterilize for 15 miii In the autoclave (6.1) set at 121 °C.
Store the solution in closed flasks/tubes at S °C (6.fl) for up to six months.
B.14 Antisera
Several types of agglutinating sera containing antibodies for one or several 0-antigens are available commercially. i.e. antisera containing one or more 0” groups (called monovalent or polyvalent anti-0 sera). anti-VI sera, and antisera containing antibodies for one or several H-factors (called monovalent or polyvalent anti-H sera).
HIS Performance testing for the quality assurance of the culture media
The definition of selectivity and productivity is specified in ISO 11133. In general, follow the procedures for performance testing described in ISO 11133. For performance testing of selective liquid media and MSRV agar, use the same inoculum volume as specified in 9.12. For MSRV agar the inoculum should contain 1O- cfu to 10 cfu for determining productivity and 10 cfu to 106 cfu for determining selectivity (see ISO 11133).